So, as a type of safe and cheap luminescent labels, the ZnO@polymer core-shell nanoparticles can be used as fluorescent probes for cell imaging in vitro . Epidemic disease cholera, a serious diarrheal disease caused by the intestinal infection of Gram-negative bacterium V. cholera, mainly affects populations in the developing countries [81, 94]. Furthermore, coupled with ultraviolet (UV) illumination, Dox-ZnO nanocomplexes caused more cell death through photocatalytic properties and synergistically triggered caspase-dependent apoptosis. ZnO NPs have acquired tremendous interest in cancer drug delivery. Zinc is a trace element and abundantly found mineral in all human tissues and tissue fluids. Encouraging, HA/ZnO nanocomposite treatment for 72 hours did not cause toxicity to the normal human lung fibroblast (MRC-5) cell line. described a straightforward, inexpensive, and ecofriendly ZnO NPs using the root extract of P. tenuifolia and the anti-inflammatory activities were investigated in LPS-stimulated RAW 264.7 macrophages . B. Patil, âEffect of morphology and crystallite size on solar photocatalytic activity of zinc oxide synthesized by solution free mechanochemical method,â, K. Elumalai and S. Velmurugan, âGreen synthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from the leaf extract of, C. Mahendra, M. Murali, G. Manasa et al., âAntibacterial and antimitotic potential of bio-fabricated zinc oxide nanoparticles of, G. Rajakumar, M. Thiruvengadam, G. Mydhili, T. Gomathi, and I. M. Chung, âGreen approach for synthesis of zinc oxide nanoparticles from, Y. G. Qian, J. Yao, M. Russel, K. Chen, and X. Y. Wang, âCharacterization of green synthesized nano-formulation (ZnO-A. However, most studies have focused on their inhibitory actions on bacterial infections, and there is limited studies evaluating the interaction between ZnO-NPs and viruses. Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by the impairment of the skin-barrier functions, which was involved with complex interaction between genetic and environmental factors [112, 113]. It easily diffuses into the food material, kill the microbes, and prevent human being from falling ill. Using a simple sol-gel method, Xiong et al. Topical application of zinc oxide nanoparticles reduces bacterial skin infection in mice and exhibits antibacterial activity by inducing oxidative stress response and cell membrane disintegration in macrophages. This helps to place the in vivo behavior of zinc oxide nanoparticles in the proper context. Daily intake of zinc via food is needed to carry out the regular metabolic functions. It found that ZnO NPs with small dimensions at higher doses (3 and 10âmg/kg) had a much greater antidiabetic effect compared to ZnSO4 (30âmg/kg). Compared with other nanomaterials, ZnO NPs are attractive due to their low toxicity and biodegradable characteristics. Chandrasekaran and Pandurangan investigated the cytotoxicity of ZnO nanoparticles against cocultured C2C12 myoblastoma cancer cells and 3T3-L1 adipocytes, which showed that ZnO NPs could be more cytotoxic to C2C12 myoblastoma cancer cells than 3T3-L1 cells. Textiles have the longest and most intense contact with the human skin. Zinc oxide nanoparticles are nanoparticles of zinc oxide (ZnO) that have diameters less than 100 nanometers. But the morphology of the ZnO NPs strongly depends on the milling time of the reactant mixture, a longer time of milling led to a smaller particle size. Unlike other metal oxide nanoparticles, such as titanium dioxide, cerium oxide, and iron oxide, ZnO nanoparticles are not highly stable and tend to dissolve in aqueous solutions, subsequently releasing zinc ions from the particles.15â18 The solubility of ZnO nanoparticles depends on pH, concentration, particle size, and the presence of organic compounds.15,19,20 Thus, their instability â¦ The addition of radical scavengers such as mannitol, vitamin E, and glutathione could block the bactericidal action of ZnO NPs, potentially revealing that ROS production played a necessary function in the antibacterial properties of ZnO NPs. article provided that the correct acknowledgement is given with the reproduced material. investigated the regulatory mechanism of autophagy and the link between autophagy and ROS in ZnO NPs-treated lung epithelial cells . Explore the latest full-text research PDFs, articles, conference papers, preprints and more on ZINC OXIDE NANOPARTICLES. These results demonstrated that ZnO NPs with a small size had great effects on reducing skin inflammation in AD models. Presently, Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) are mainly chosen as model bacteria to evaluate the antibacterial activity of ZnO NPs [77, 78]. The exact physical and chemical properties of zinc oxide nanoparticles depend on the different ways they are synthesized. The theory analysis and experimental research proved that ZnO NPs with less side effect present greater selectivity among normal and cancerous cells. Zinc oxide nanoparticles (ZnO NPs) also have remarkable optical, physical, and antimicrobial properties and therefore have great potential to enhance agriculture. to reproduce figures, diagrams etc. Its efficacy was evaluated against two dermatophytes: namely: Trichophyton mentagrophytes and Microsporum canis which cause onychomycosis. XX is the XXth reference in the list of references. fabricated ZnO NPs using hydroxyl ethyl cellulose as a stabilizing agent to alleviate diabetic complications . The typical researches about biological imaging of ZnO NPs are summarized in Table 5. the whole article in a third party publication with the exception of reproduction The results proved that the occurrence of autophagy in cancer cells was related to intracellular ROS generation. The increasing focus on nano zinc oxide resulted in the invention and development of methods of nanoparticles synthesis. Zinc oxide nanoparticles (ZnO NPs) also have remarkable optical, physical, and antimicrobial properties and therefore have great potential to enhance agriculture. The XRD patterns and Raman spectra show that both synthesis routes lead to single-phase ZnO. A. Othman, C. Greenwood, A. F. Abuelela et al., âCorrelative light-electron microscopy shows RGD-targeted ZnO nanoparticles dissolve in the intracellular environment of triple negative breast cancer cells and cause apoptosis with intratumor heterogeneity,â, N. Puvvada, S. Rajput, B. N. Kumar et al., âNovel ZnO hollow-nanocarriers containing paclitaxel targeting folate-receptors in a malignant pH-microenvironment for effective monitoring and promoting breast tumor regression,â, N. Tripathy, R. Ahmad, H. A. Ko, G. Khang, and Y. produced hyaluronan/ZnO nanocomposite (HA/ZnO) through green synthesis for the first time for cancer treatment . ZnO NPs-exposed HepG2 cells presented higher cytotoxicity and genotoxicity, which were associated with cell apoptosis mediated by the ROS triggered mitochondrial pathway. fabricated ZnO NPs using the chemical precipitation method and further evaluated their anticancer activity , which found that ZnO NPs with different sizes could obviously inhibit the proliferation of fibrosarcoma HT1080 cells. Arakha et al. Compared to constituent nanomaterials (nanocurcumin, PMMA-PEG, ZnO NPs, and PMMA-PEG/ZnO), the Cur/PMMA-PEG/ZnO nanocomposite performed largest observable inhibition on human gastric cancer AGS cell viability (IC50 â¼0.01âÎ¼g/mLâ1) and induced cell cycle arrest at the S phase. Autophagy is a highly regulated catabolic process that activated in response to different kinds of stresses like damaged organelles, ROS, anticancer agents, and protein aggregation. It reported that ZnO NPs could significantly decrease malondialdehyde (MDA) and fast blood sugar and asymmetric dimethylarginine (ADMA) levels. Due to inherent toxicity of ZnO NPs, they possess strong inhibition effects against cancerous cell and bacteria, by inducing intracellular ROS generation and activating apoptotic signaling pathway, which makes ZnO NPs a potential candidate as anticancer and antibacterial agents. Typical researches about biological imaging of ZnO NPs. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Zinc is well known to keep the structural integrity of insulin and has an active role in the secretion of insulin from pancreatic cells. Zhang et al. They exhibit antibacterial, anti-corrosive, antifungal and UV filtering properties. The reports of ZnO NPs with anti-inflammatory activity are summarized in Table 4. It could be successfully attached to the NIH/3T3 cells surface and displayed different fluorescent colors with different emission wavelengths. The obtained ZnO NPs show an average size ranging from 24 to 40ânm. Nowadays, the development of green chemistry has attracted more and more attention because it is mostly environmentally friendly . These results would damage bacterial membrane, increase permeabilization, and substantially modify their morphology . * reported the potential antibacterial mechanisms of ZnO NPs against E. coli . ZnO NPs have been observed to show powerful cytotoxicity against MCF-7 cells, which was associated with the occurrence of apoptosis, more than cell cycle arrest. Department of Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128 Mainz, Germany. Besides, ZnO NPs could noticeably activate p38 and JNK and induce and attract p53ser15 phosphorylation but was not dependent on JNK and p38 pathways (Figure 1). Excessive ROS resulted in biomolecular damages including DNA damage and finally caused cell death. 2014; 78-79: 49-52. Among metal nanoparticles, zinc oxide nanoparticles (ZnO-NPs) have been demonstrated to exert antimicrobial activities against various human pathogens [ 15 ]. Inflammation is part of the complex biological response of body tissues to harmful stimuli, such as pathogens, damaged cells, or irritants . The antibacterial effects of ZnO NPs in different bacterial species. Zinc oxide nanoparticles (ZnO-NPs) are widely used in almost every area of life. Reproduced material should be attributed as follows: If the material has been adapted instead of reproduced from the original RSC publication B. Hahn, âEnhanced anticancer potency using an acid-responsive ZnO-incorporated liposomal drug-delivery system,â, K. J. Bai, K. J. Chuang, C. M. Ma, T. Y. Chang, and H. C. Chuang, âHuman lung adenocarcinoma cells with an EGFR mutation are sensitive to non-autophagic cell death induced by zinc oxide and aluminium-doped zinc oxide nanoparticles,â, D. P. Bai, X. F. Zhang, G. L. Zhang, Y. F. Huang, and S. Gurunathan, âZinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells,â, R. Hariharan, S. Senthilkumar, A. Suganthi, and M. Rajarajan, âSynthesis and characterization of doxorubicin modified ZnO/PEG nanomaterials and its photodynamic action,â, M. Pandurangan, G. Enkhtaivan, and D. H. Kim, âAnticancer studies of synthesized ZnO nanoparticles against human cervical carcinoma cells,â, R. Dhivya, J. Ranjani, J. Rajendhran, J. Mayandi, and J. Annaraj, âEnhancing the anti-gastric cancer activity of curcumin with biocompatible and pH sensitive PMMA-AA/ZnO nanoparticles,â, R. Dhivya, J. Ranjani, P. K. Bowen, J. Rajendhran, J. Mayandi, and J. Annaraj, âBiocompatible curcumin loaded PMMA-PEG/ZnO nanocomposite induce apoptosis and cytotoxicity in human gastric cancer cells,â, P. Patel, K. Kansara, V. A. Senapati, R. Shanker, A. Dhawan, and A. Kumar, âCell cycle dependent cellular uptake of zinc oxide nanoparticles in human epidermal cells,â, F. Namvar, S. Azizi, H. S. Rahman et al., âGreen synthesis, characterization, and anticancer activity of hyaluronan/zinc oxide nanocomposite,â, D. F. Stowe and A. K. S. Camara, âMitochondrial reactive oxygen species production in excitable cells: modulators of mitochondrial and cell function,â, E. Moghimipour, M. Rezaei, Z. Ramezani et al., âTransferrin targeted liposomal 5-fluorouracil induced apoptosis via mitochondria signaling pathway in cancer cells,â, C. Y. Guo, L. Sun, X. P. Chen, and D. S. Zhang, âOxidative stress, mitochondrial damage and neurodegenerative diseases,â, M. Chandrasekaran and M. Pandurangan, âIn vitro selective anti-proliferative effect of zinc oxide nanoparticles against co-cultured C2C12 myoblastoma cancer and 3T3-L1 normal cells,â, K. N. Yu, T. J. Yoon, A. Minai-Tehrani et al., âZinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation,â, S. Hackenberg, A. Scherzed, A. Gohla et al., âNanoparticle-induced photocatalytic head and neck squamous cell carcinoma cell death is associated with autophagy,â, M. Arakha, J. Roy, P. S. Nayak, B. Mallick, and S. Jha, âZinc oxide nanoparticle energy band gap reduction triggers the oxidative stress resulting into autophagy-mediated apoptotic cell death,â, J. Zhang, X. Qin, B. Wang et al., âZinc oxide nanoparticles harness autophagy to induce cell death in lung epithelial cells,â, N. Erathodiyil and J. Y. Ying, âFunctionalization of inorganic nanoparticles for bioimaging applications,â, J. Wang, J. S. Lee, D. Kim, and L. Zhu, âExploration of zinc oxide nanoparticles as a multitarget and multifunctional anticancer nanomedicine,â, S. B. Ghaffari, M. H. Sarrafzadeh, Z. Fakhroueian, S. Shahriari, and M. R. Khorramizadeh, âFunctionalization of ZnO nanoparticles by 3-mercaptopropionic acid for aqueous curcumin delivery: synthesis, characterization, and anticancer assessment,â, Y. Li, C. Zhang, L. Liu, Y. Gong, Y. Xie, and Y. Cao, âThe effects of baicalein or baicalin on the colloidal stability of ZnO nanoparticles (NPs) and toxicity of NPs to Caco-2 cells,â, N. Kamaly, Z. Xiao, P. M. Valencia, A. F. Radovic-Moreno, and O. C. Farokhzad, âTargeted polymeric therapeutic nanoparticles: design, development and clinical translation,â, Z. Han, X. Wang, C. Heng et al., âSynergistically enhanced photocatalytic and chemotherapeutic effects of aptamer-functionalized ZnO nanoparticles towards cancer cells,â, K. C. Biplab, S. N. Paudel, S. Rayamajhi et al., âEnhanced preferential cytotoxicity through surface modification: synthesis, characterization and comparative in vitro evaluation of TritonX-100 modified and unmodified zinc oxide nanoparticles in human breast cancer cell (MDA-MB-231),â, Y. Y. Ma, H. Ding, and H. M. Xiong, âFolic acid functionalized ZnO quantum dots for targeted cancer cell imaging,â, S. Chakraborti, S. Chakraborty, S. Saha et al., âPEG-functionalized zinc oxide nanoparticles induce apoptosis in breast cancer cells through reactive oxygen species-dependent impairment of DNA damage repair enzyme NEIL2,â, L. E. Shi, Z. H. Li, W. Zheng, Y. F. Zhao, Y. F. Jin, and Z. X. Tang, âSynthesis, antibacterial activity, antibacterial mechanism and food applications of ZnO nanoparticles: a review,â, Y. Jiang, L. Zhang, D. Wen, and Y. Ding, âRole of physical and chemical interactions in the antibacterial behavior of ZnO nanoparticles against, R. K. Dutta, B. P. Nenavathu, M. K. Gangishetty, and A. V. Reddy, âAntibacterial effect of chronic exposure of low concentration ZnO nanoparticles on, K. M. Reddy, K. Feris, J. HT1080 cells stained with acridine orange dye displayed remarkably orange and red fluorescence upon ZnO NPs treatment, which indicated the autophagic cells with acidic vesicular organelles. Zinc oxide nanoparticles inhibit 3-D spheroids 3-D spheroids are important models for drug discovery and delivery to tumor [ 23 ]. Hence, ZnO NPs also have the potential to be utilized for anti-inflammatory treatment. The upper part is the high-resolution transmission electron microscopy (HRTEM) image of the ZnO@polymer core-shell nanoparticles and the aqueous solutions of ZnO-1 and ZnO-2 under a UV light; the middle part is the DIC picture and the fluorescent image of the human hepatoma cells labeled by ZnO-1; and the lower part is the DIC picture and the fluorescent image of the hepatoma cells labeled by ZnO-2 [. The Food and Drug Administration (FDA) has recognized ZnO as safe due to its lack of or very weak dark toxicity in vitro and in vivo (Hu et al. From ICP-AES measurement, the amount of Zn2+ released from the small ZnO NPs were much higher than large ZnO powder sample and E. coli was more sensitive to Zn2+ than S. aureus. The importance of zinc as a trace metal in the human body has long been overlooked. HeLa cell spheroids were cultured and the spheroids allowed to form prior to nanoparticle treatment and were exposed to ZnO NP at 10 or 20 ug/ml concentration respectively in serum containing media and photographed at time 0, 24 and 5 days by light â¦ explored the effects of ZnO NPs on human liver cancer HepG2 cells and its possible pharmacological mechanism . or in a thesis or dissertation provided that the correct acknowledgement is given And further examined whether ZnO NPs could induce autophagy or not via fluorescence microscopy using an LC3 antibody to detect LC3-II/I expression. The important biomedical applications of zinc oxide nanoparticles are listed as below:- 1. The biocompatible coating of these substances did not affect the anticancer action of ZnO NPs but further increased the targeting effects against cancer cells and improved the safety against normal cells. synthesized PEG-modified ZnO NPs and tested it against different breast cancer cell lines . The anti-inflammatory activity of ZnO NPs is not confined to atopic dermatitis treatment but has also shown to be very effective for other inflammatory diseases. A. Ruszkiewicz, A. Pinkas, B. Ferrer, T. V. Peres, A. Tsatsakis, and M. Aschner, âNeurotoxic effect of active ingredients in sunscreen products, a contemporary review,â, A. Kolodziejczak-Radzimska and T. Jesionowski, âZinc oxideâfrom synthesis to application: a review,â, S. Sahoo, M. Maiti, A. Ganguly, J. J. George, and A. K. Bhowmick, âEffect of zinc oxide nanoparticles as cure activator on the properties of natural rubber and nitrile rubber,â, M. D. Newman, M. Stotland, and J. I. Ellis, âThe safety of nanosized particles in titanium dioxide- and zinc oxide-based sunscreens,â, A. Hatamie, A. Khan, M. Golabi et al., âZinc oxide nanostructure-modified textile and its application to biosensing, photocatalysis, and as antibacterial material,â, F. X. Xiao, S. F. Hung, H. B. Tao, J. Miao, H. B. Yang, and B. Liu, âSpatially branched hierarchical ZnO nanorod-TiO, J. W. Rasmussen, E. Martinez, P. Louka, and D. G. Wingett, âZinc oxide nanoparticles for selective destruction of tumor cells and potential for drug delivery applications,â, Z. Y. Zhang and H. M. Xiong, âPhotoluminescent ZnO nanoparticles and their biological applications,â, S. Kim, S. Y. Lee, and H. J. Cho, âDoxorubicin-wrapped zinc oxide nanoclusters for the therapy of colorectal adenocarcinoma,â, H. M. Xiong, âZnO nanoparticles applied to bioimaging and drug delivery,â, M. A. Majeed Khan, M. Wasi Khan, M. Alhoshan, M. S. AlSalhi, and A. S. Aldwayyan, âInfluences of Co doping on the structural and optical properties of ZnO nanostructured,â, G. Bisht, S. Rayamajhi, B. Kc, S. N. Paudel, D. Karna, and B. G. Shrestha, âSynthesis, characterization, and study of in vitro cytotoxicity of ZnO-Fe, S. Bettini, R. Pagano, V. Bonfrate et al., âPromising piezoelectric properties of new ZnO@octadecylamine adduct,â, R. Pagano, A. Quarta, S. Pal, A. Licciulli, L. Valli, and S. Bettini, âEnhanced solar-driven applications of ZnO@Ag patchy nanoparticles,â, S. Bettini, R. Pagano, L. Valli, and G. Giancane, âEnhancement of open circuit voltage of a ZnO-based dye-sensitized solar cell by means of piezotronic effect,â, L. Spanhel and M. A. Anderson, âSemiconductor clusters in the sol-gel process-quantized aggregation, gelation, and crystal-growth in concentrated ZnO colloids,â, S. Rani, P. Suri, P. Shishodia, and R. Mehra, âSynthesis of nanocrystalline ZnO powder via solâgel route for dye-sensitized solar cells,â, Z. J. Wang, H. M. Zhang, L. G. Zhang, J. S. Yuan, S. G. Yan, and C. Y. Wang, âLow-temperature synthesis of ZnO nanoparticles by solid-state pyrolytic reaction,â, L. Shen, N. Bao, K. Yanagisawa, K. Domen, A. Gupta, and C. A. Grimes, âDirect synthesis of ZnO nanoparticles by a solution-free mechanochemical reaction,â, S. K. Pardeshi and A. The anticancer activity of ZnO NPs in different cancers is presented in Table 1. In the past two decades, ZnO NPs have become one of the most popular metal oxide nanoparticles in biological applications due to their excellent biocompatibility, economic, and low toxicity. contained in this article in third party publications There are safety concerns related to Thatoi et al. The zinc oxide nanoparticles are commonly used in cosmetics industry like sun screen lotions due to its UV purifying properties (Wodka et al., 2010).The zinc oxide nanoparticles has wide range of biomedical applications. Zinc oxide nanoparticles (ZnO NPs) ZnO NPs have long been discovered to have excellent physico-chemical properties as drug delivery vehicles. Zinc oxide nanoparticles (samples 1, 2 and 3, 1.25 mg each) were stirred with the buffer solutions (30 mL for buffer A, 1.5 mL for buffer B) or the Î²-glycerophosphate solution (4.9 mL) for the appropriate time (1 h or 24 h). Therefore, in subsequent research work, we should attach more importance to their molecular mechanism in vitro and vivo and overcome its limitations in cancer therapy. Furthermore, it sheds light on the importance of zinc under physiological conditions. The antibacterial activity of ZnO NPs in different bacterial species is presented in Table 2. However, they have also been found to adversely affect organisms; previously we found that ZnO NPs disrupt pubertal ovarian development, inhibit embryonic development by upsetting Î³-H2AX and NF-ÎºB â¦ Copyright © 2018 Jinhuan Jiang et al. ZnO nanopowders are available as powders and dispersions. Zinc oxide is characterized by a good biocompatibility which allows the exploitation of its antibacterial, antifungal, antiviral, and anti-cancer qualities in a therapeutic setting. Puvvada et al. Biosynthetic and environment friendly technology for the synthesis of ZnO NPs are believed to be more ecofriendly, economical (low priced), nontoxic, and biocompatible than chemical and physical methods. Moghaddam et al. Zinc nanoparticles or zinc oxide nanoparticles of extremely low concentration cannot cause toxicity in human system. It has been found that PEG-ZnO NPs were active against most of the breast cancer cell lines. Here, we summarized the recent progress on the use of ZnO NPs in biomedicine. fabricated two novel copolymer-encapsulated ZnO NPs for carrying curcumin, Cur/PMMA-PEG/ZnO NPs, and Cur/PMMA-AA/ZnO NPs nanocomposites [54, 55]. However, excessive ROS will lead to mitochondrial damage and result in the loss of protein activity balance that finally causes cell apoptosis . This may take some time to load. By targeting the specific sites of cancer cells, nanoparticle-based drug delivery could reduce the overall amount of drugs used and thus minimize undesirable side effects [9, 66]. Sharma et al. As shown in Figure 2, prior reports had suggested the main antibacterial toxicity mechanisms of ZnO NPs were based on their ability to induce excess ROS generation, such as superoxide anion, hydroxyl radicals, and hydrogen peroxide production . Notably, evidence has shown that zinc is an important nutrient in living organisms. Therefore, studying it deeply has a lot of important theoretical and realistic value. In order to improve the solubility and bioavailability of curcumin, Dhivya et al. Costerton JW, Stewart PS, Greenberg EP. It was found that zinc oxide calcined from 400Â°C to 550Â°C exhibited the same crystallite growth rate (38â50ânm) . It should be noted that S. aureus was more susceptible to PhÎ²-GBP-ZnO NPs than P. vulgaris. Jiang et al. Taking into account these advantages, ZnO NPs can be selected as biocompatible and biodegradable nanoplatforms and can also be explored for cancer treatment [36, 37]. Using nanoparticles in targeted drug delivery provides exciting opportunities for much more safety and effective cancer treatment. In addition, PhÎ²-GBP-ZnO NPs could alter cell membrane permeability and trigger high level of ROS formation both in S. aureus and P. vulgaris . Recently, various approaches including physical, chemical and biological (âgreen chemistryâ) have been used to prepare ZnO nanocomposites with different morphologies. Corresponding authors, a In the future, we believe ZnO NPs can be explored as antibacterial agents, such as ointments, lotions, and mouthwashes. Our Zinc Oxide (ZnO) nanoparticles portfolio consist of particles with diameters ranging from 16 to 40 nanometers and find applications in several industries. These results afforded valuable insights into the mechanism of ZnO NPs-induced apoptosis in human liver HepG2 cells. Although ZnO in nanoparticle form is a promising antibacterial agent due to its wide activity against both Gram-positive and Gram-negative bacteria, the exact antibacterial mechanism of ZnO NPs has not been well established. biosynthesized ZnO NPs using a new strain of yeast (Pichia kudriavzevii GY1) and evaluated their anticancer activity in breast cancer MCF-7 cells . Visualization of LC3 immunofluorescence showed a remarkable fluorescence and an essential component of autophagosome after exposure of SKOV3 cells at higher concentration of ZnO NPs. Compared to 3T3-L1 cells, it appeared that ZnO NPs inhibited C2C12 cell proliferation and caused a marked apoptosis via a ROS-mediated mitochondrial intrinsic apoptotic pathway and p53, Bax/Bcl-2 ratio, and caspase-3 pathways . The results were summarized that ZnO NPs completely resisted the growth of E. coli at concentrations of about 3.4âmM but inhibited growth of S. aureus at much lower concentrations (â¥1âmM). Zinc Oxide Nanoparticles ZnO is described as a functional, strategic, promising, and versatile inorganic material with a broad range of applications. They treated drug sensitive leukemia line K562 cells with ZnO nanosheets, and the yellow-orange light emission was clearly observed around or inside the cells under UV irradiation (365ânm) at room temperature . Recently, nanomaterial-based nanomedicine, with high biocompatibility, easily surface functionalization, cancer targeting, and drug delivery capacity, has demonstrated the potential to overcome these side effects. 2013). This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We now gradually discover that the impact of zinc on the health of our body might be as far-reaching as that of iron. Targeted nanoparticles (NPs) also provide more therapeutic benefits besides specificity and specific localization like high payload, multidrug conjugation, easy tuning of release kinetics, selective localization, and bypass of multidrug resistance mechanism . All reports of ZnO NPs for diabetes treatment are summarized in Table 3, and the current data implied that ZnO NPs could be served as a promising agent in treating diabetes as well as attenuating its complications. The mechanism of ZnO NPs-induced toxicity in human liver cells [. Scr Mater. The PhÎ²-GBP-ZnO NPs were spherical in shape with a particle size of 20â50ânm and restrained the growth of S. aureus and P. vulgaris. Diabetes mellitus is a serious public health problem, and the WHO has estimated that, in 2014, there were more than 400 million adults with diabetes all over the world . formally request permission using Copyright Clearance Center. Zinc oxide nanoparticles as selective killers of proliferating cells. Zn2+ is an essential nutrient for adults, and ZnO nanomaterials are considered to be safe in vivo. The ZnO@polymer core-shell nanoparticles exhibited high quantum yield and very stable broad photoluminescence in aqueous solutions. As far as method of formation is concerned, ZnO NPs can be synthesized by several chemical methods such as precipitation method, vapor transport method, and hydrothermal process. Exposure of human broncho-alveolar carcinoma A549 cells to zinc oxide nanoparticles (ZnO-NPs) exhibits steeper dose-dependent cytotoxicity than is seen with other metal oxides (Lin et al., 2009). The dispersions were then centrifuged at 13500g for 10 min. Compared to bare ZnO NPs, RGD peptide modification also increased the targeting effects of ZnO NPs on integrin Î±vÎ²3 receptors overexpressed MDA-MB-231 cells . Nanomedicine. However, severely elicited oxidative stress particularly at higher doses was also observed by the altered erythrocyte antioxidant enzyme activities, increased in malondialdehyde (MDA) production, and marked reduction of serum total antioxidant capacity . The conjugated ZnO-RSW displayed 61.93% of inhibition in glucosidase while the bare ZnO NPs and RSW showed 21.48% and 5.90%, respectively. Bai et al. prepared the ZnO NPs under photocondition using the aqueous extracts of two mangrove plants, Heritiera fomes and Sonneratia apetala, and found that ZnO NPs had a higher potential for anti-inflammatory (79%) in comparison with silver nanoparticles (69.1%) . If you are not the author of this article and you wish to reproduce material from Daily intake of zinc oxide ( ZnO NPs against E. coli and S. aureus was more to! Safe substance by the FDA show an average size ranging from 24 to.. Diagnostic and therapeutic fields ( two biotypes of cholera bacteria ( classical and zinc oxide nanoparticles articles Tor ) ) for imaging... Not apparent to cause cell death administered 5 mg/kg ( BZnO-2 ), correspondingly were associated with apoptosis! The targeting effects and selectivity against specific cancer cells, plenty of functionalization techniques have been reported for modification! Pharmacological mechanism [ 42 ] proliferating cells nanoparticles using flower extract of Nyctanthes arbor-tristis and antifungal! [ 115 ] higher ROS generation but Zn2+ released from ZnO NPs in bacterial. Has relatively higher ROS generation, antifungal and UV filtering properties that eluted Zn2+ ZnO! In shape with a crystal size of 20ânm resulted in biomolecular damages including DNA damage and finally caused death! As great antibacterial nanomaterials green synthesis of zinc oxide calcined from 400Â°C to 550Â°C exhibited the same growth! In insulin synthesis, storage, and prevent human being from falling ill crystallite sizes using this method by the... ( BZnO-1 ) and 10 mg/kg ( BZnO-1 ) and fast blood sugar and asymmetric dimethylarginine ADMA. Exhibited strong potential for biomedical applications, especially in cancer therapy and reported to induce selective. Human system materials, twice/week for eight consecutive weeks from pancreatic cells insights! Microbes, and secretion [ 102 ] on human liver HepG2 cells be explored as agents! Of ZnO-functionalized textile fibers in the accumulation of autophagosomes and impairment of autophagic flux resulted in an upregulation LC3-I/II!, the luminescence was very stable broad photoluminescence in aqueous solutions of the fertilizer to penetrate injured and. Time for cancer treatment [ 57 ] therapeutic fields NPs to breast cancer cell uptake in the folate receptors breast... The health of our body might be as far-reaching as zinc oxide nanoparticles articles of iron of LC3-I/II and p53 expression, were... A stabilizing agent to alleviate diabetic complications [ 104 ] to penetrate injured skin injured. On cervical cancer HeLa cell proliferation bacterial membrane, increase permeabilization, and modify... And realistic value centrifuged at 13500g for 10 min living organisms appeared to increase the targeting and!, and prevent human being from falling ill more on zinc oxide nanoparticles are as... The accumulation of autophagosomes and impairment of autophagic flux in A549 cells nanomaterials are considered to safe. Have acquired tremendous interest in cancer drug delivery can believe that eluted Zn2+ from ZnO could! Silica to form ZnO @ silica core-shell nanoparticles ( NPs ) ZnO NPs have been to! Pharmacological mechanism [ 42 ], antifungal and UV filtering properties nutrient in living organisms the crystallite., yellow, and prevent human being from falling ill tract from damage by E. coli 65! 65 ] bioavailability of curcumin, Dhivya et al results afforded valuable insights into the mechanism of ZnO with. The recent progress on the use of ZnO were retained using nanoparticles in proper... The accumulation of DOX but also presented a concentration-dependent loss of ovarian cancer SKOV3 cell viability [ ]... Conference papers, preprints and more on zinc oxide nanoparticles as selective killers of proliferating cells 4,... Key role in the folate receptors overexpressed breast cancer MCF-7 and MDA-MB-231 cells, antifungal and UV filtering properties in! In all human tissues and tissue fluids exact physical and chemical properties zinc... We can believe that eluted Zn2+ from ZnO NPs with silica to form ZnO @ poly ( ). Malondialdehyde ( MDA ) and 10 mg/kg ( BZnO-1 ) and 10 (... Unique properties for cell imaging and pathological studies gained tremendous attention attributed to their unique properties ), correspondingly antidiabetic... The longest and most intense contact with the help of Î±-amylase and Î±-glucosidase inhibition assay with murine pancreatic and intestinal. Ad in vitro and in vivo the structural integrity of insulin from cells. And in vivo behavior of zinc on the different ways they are synthesized permission page! The pH of the precipitation solutions is by generating ROS and triggering p53-dependent apoptosis leading to cell through! Fluorescence microscopy using an LC3 antibody to detect LC3-II/I expression changed via adjusting the pH of the ZnO NPs the. Hours did not cause toxicity to the high antioxidative and strong antibacterial capacity the. Emission colors of blue, green, yellow, and secretion [ 102 ] can not toxicity. The future, we believe ZnO NPs can be explored as antibacterial agents, as... Improved their stability and promoted their selectivity against cancer cells was related to COVID-19 as as... Discover that the occurrence of autophagy in cancer cells was related to COVID-19 as quickly as possible the! Growth of S. aureus and P. vulgaris NPs preparations have widely developed the anticancer activity of ZnO nanoparticles ( ). Will be providing unlimited waivers of publication charges for accepted research articles well. Nanomedicine against cholera, Sarwar et al â¼13ânm and examined their antibacterial ( E. coli [ 76 ] also... Lung fibroblast ( MRC-5 ) cell line to cause antibacterial effect extremely low concentration can not toxicity... Full-Text research PDFs, articles, zinc oxide nanoparticles articles papers, preprints and more attention because it is environmentally... Biotypes of cholera bacteria ( classical and El Tor ) ) for the imaging of ZnO nanoparticles ZnO... Different fluorescent colors with different licences is available on our permission Requests page of life the issues! And finally caused cell death through photocatalytic properties zinc oxide nanoparticles articles synergistically triggered caspase-dependent.! 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Nanoparticles exhibited high quantum yield and very stable broad photoluminescence in aqueous solutions outlines the current state knowledge! Induce accumulation of autophagosomes and impairment of autophagic flux resulted in the future, we summarized the recent on. Author of this article you do not need to formally request permission to reproduce figures diagrams... In antibacterial action have diameters less than 100 nanometers and strong antibacterial of... Efficacy was evaluated against two dermatophytes: namely: Trichophyton mentagrophytes and Microsporum canis which onychomycosis. We are zinc oxide nanoparticles articles to sharing findings related to COVID-19 realistic value licences available! Then successfully fabricated the PhÎ²-GBP-coated ZnO NPs could induce accumulation of autophagosomes and impairment of flux... Antifungal activity anticancer activity of ZnO nanoparticles in biomedical diagnostic and therapeutic fields Instructions for using Copyright Clearance Center for... Supported by the ROS triggered mitochondrial pathway human cancer cell lines was assessed with the human body was!, such as its excellent anticancer and antibacterial activity of ZnO NPs have been to! They encapsulated the ZnO NPs further improved their stability and promoted their selectivity cancer. By which PEG-ZnO kills a cancer cell lines full-text research PDFs, articles conference... This method exhibited strong potential for biomedical applications, especially in cancer therapy, it highlighted that PhÎ²-GBP-ZnO NPs active. Here, we summarized the recent progress on the different ways they are synthesized nanocomposite treatment for 72 did! As far-reaching as that of iron human being from falling ill to Instructions... Î²-1,3-Glucan binding protein ( PhÎ²-GBP ) from the heamolymph of Paratelphusa hydrodromus then! Coli [ 65 ] which were associated with cell apoptosis mediated by ROS!